Humanparainfluenzavirus1拉丁名
ATCCVR-94编号
ClassificationParamyxoviridae,Respirovirus
DepositedAsParainfluenza1
AgentHumanparainfluenzavirus1
StrainC35
Applications
Respiratoryresearch
BiosafetyLevel安全等级2
BiosafetyclassificationisbasedonU.S.PublicHealthServiceGuidelines,itistheresponsibilityofthecustomertoensurethattheirfacilitiescomplywithbiosafetyregulationsfortheirowncountry.
ProductFormat提供形式frozen1mLpervial
StorageConditions-70°Corcolder
CommentsAdditionof1μg/mLtrypsin(1:250Gibcotrypsincatalog#27250018)toculturemediumisrequiredforoptimalgrowth.AgglutinatesbothchickenandguineapigRBC(0.5%)butthelattertoahighertiterthantheformer.Basedonelectronmicroscopicevidence,thisproductmayalsocontainanasyetunidentifiedpolyomavirus.
ViruspresencecanbedetectedwithMillipore#5019Parainfluenza1AntibodyFITCReagent
EffectonHost
SlightCPE,roundingandsloughing
RecommendedHost
LLC-MK2cells(ATCCCCL-7.1)
GrowthConditions生长条件
Temperature培养温度:37°C
RecommendationsForInfection:Forbestresults,infectionshouldbeperformedona80-90%confluent,18-48houroldcellularmonolayer.Preparedilutionofvirusinminimumamountofvolume(e.g.1mLper25cm2).WashmonolayerwithPBSorserumfreemediumpriortoinoculation.Adsorbvirusdilutionfor1-2hoursat37°Cinahumidified5%CO2atmosphere,rockingevery20-30minutestoredistributeinoculum.Endadsorptionbyaddingvirusgrowthmedium.
Incubation:5-7dayscontinuouslyrockingat37°Cinahumidified5%CO2atmosphere.
EffectonHost
SlightCPE,roundingandsloughing
NameofDepositorRHuebner,RMChanock,JCHierholzer,AChappell
SourceThroatswab,3-year-oldboywithacutelaryngitis,Washington,DC,1957
References参考文献
AndrewesCH,etal.Para-influenzaviruses1,2,and3:Suggestednamesforrecentlydescribedmyxoviruses.Virology8:129-130,1959.PubMed:13669331
ChanockRM..N.Engl.J.Med.258:207-213,1958.
CrossReferences
Nucleotide(GenBank):JQ901971.1Humanparainfluenzavirus1strainATCCVR-94,partialgenome
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