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S1A(Thy-1-b)

S1A(Thy-1-b)

  • 平台编号: zl-058200
  • 规格: frozen
  • 细胞信息: S1A(Thy-1-b)
  • 关注度:7

运输方式:冻存运输
ATCC Number:TIB-231?
相关疾病:淋巴瘤
数量:大量
细胞形态:淋巴样
细胞类型:T淋巴细胞
是否是肿瘤细胞:0
物种来源:小鼠
生长状态:悬浮生长
品系:BALB/c
规格:0.5mg Designations: S1A(Thy-1-b)
Depositors: ?R Hyman
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast


Source: Disease: lymphoma
Strain: BALB/c
Cell Type: T lymphocyte;
Cellular Products:The cells secrete a mutant Thy-1 glycoprotein.
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:This line is a Class B Thy-1 negative (Thy-1-) mutant derived from S1A.TB.4.8.2 (ATCC TIB-27 ).
The mutation affects the biosynthesis and/or addition of the glycophospholipid anchor.
The line may be useful in studies of the synthesis and assembly of glycophospholipid linked membrane proteins.
The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; heat-inactivated horse serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1807: Conzelmann A, et al. No glycolipid anchors are added to Thy-1 glycoprotein in Thy-1-negative mutant thymoma cells of four different complementation classes. Mol. Cell. Biol. 8: 674-678, 1988. PubMed: 2895421

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