电话咨询
400-009-7996
快速送货
顺丰快捷出单
MG1655(DE3) SEGA066

MG1655(DE3) SEGA066

  • 平台编号: zl-072107
  • 规格: K12/
  • 拉丁属名: Escherichia coli
  • 关注度:7

  Species:Escherichia coli

  Group:K12

  Strain designation:MG1655(DE3)SEGA066

  Accession number:LMBP 12902

  Depositor:A.Ehrmann1 and Prof.Dr M.N?rholm1.It was constructed by C.Bayer1,A.Ehrmann1 and M.Rennig1.

  1 The Novo Nordisk Foundation Center for Biosustainability,Technical University of Denmark,Horsholm,Denmark

  Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

  Other culture collection numbers:SEGA 066

  Medium:LB-Lennox

  Selection marker:tetracycline(10?g/ml)

  Temperature:28°C

  Genotype:F-λ(DE3[lacI lacUV5-T7gene1 ind1 sam7 nin5])ilvG-rfb-50 rph-1 glmS-pstS::PT7-medium-PROTi-gfp-tetA(Source:http://openwetware.org/wiki/E._coli_genotypes#MG1655+Bayer et al.,2021)

  SEGA sequence:p12902.gb

  Phenotype:tetR

  Properties:The strain was created based on Escherichia coli K12 MG1655,by integrating the SEGA landing pad between the glmS and pstS loci.The strain contains DE3,aλprophage carrying the T7 RNA polymerase gene under control of the lac UV5 promoter and lacIq.IPTG is required to induce expression of the T7 RNA polymerase.Protein expression from transforming plasmids containing a T7 promoter-driven expression system is repressed until IPTG induction of T7 RNA polymerase expression occurs.

  Further information:This host strain is intended for simplistic bacterial genome engineering,using the SEGA genome architecture.

  Original reference:Bayer et al.,Nat.Commun.12(2021),5876[DOI:10.1038/s41467-021-26155-5]

  Restricted use:-BCCM MTA

  -The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.

  Refer in your Materials and Methods:

  Escherichia coli K12 MG1655(DE3)SEGA066(LMBP 12902)is available at BCCM/GeneCorner.It was deposited by A.Ehrmann and Prof.Dr M.N?rholm and was published in Bayer et al.,2021.

 如需发生退货:
  ①注明退货原因,如果产品存在质量问题,请务必说明;
  ②确保产品及配件、礼品或者赠品、发票(若有)、本商城产品说明书齐全;请于购买产品后妥善保管购物发票(若有)及产品的说明书;
  ③如果组合产品中有部分产品存在质量问题,在您办理退货时,必须提供完整的组合产品,不支持单独退货;
  ④另外,任何因用户非正常使用及保管导致出现质量问题的产品,怒不退换,请用户注意并谅解;
  ⑤如因用户原因无法退回发票(若有)或产品说明书的,可能造成我们无法为您办理退换货事宜。
  如需发生换货
  ①发生换货时,非组合产品只能更换同一系列产品(同系列产品的不同特性、规格的价格差异实行多退少补),不提供不同系列产品的换货服务;
  ②组合商品可以部分或全部换货,但只能更换与原产品同系列的商品(同系列商品的不同特性、规格的价格差异实行多退少补),不提供不同系列组合产品的更换服务。
  注意:因网站所供应为特殊物质,非产品质量问题一律不允许退换,请悉知!(培养物一经发出请在售后期内处理)谢谢您的支持!